sirt2 polyclonal antibody (Proteintech)

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Proteintech sirt2 polyclonal antibody
Sirt2 Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 325 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sirt2+polyclonal+antibody/SIRT1+Antibody/pm41638270-69-176-179
Average 96 stars, based on 325 article reviews

sirt2 polyclonal antibody - by Bioz Stars, 2026-10

96/100 stars

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Western Blot:

Article Title: K92 Lactylation of YBX1 orchestrates m5C RNA recognition to facilitate colorectal Cancer progression.
Article Snippet: RNA 5-methylcytosine (m5C) methylation is a vital epitranscriptomic mark involved in regulating numerous cellular and disease processes.. A central effector of m5C-modified transcripts is Y-box binding protein 1 (YBX1), a key RNA-binding factor that modulates multiple aspects of RNA metabolism.. Here, we identify lysine 92 (K92) as a predominant and previously unrecognized site of lactylation on YBX1, installed by the acyltransferase p300 and erased by the delactylase SIRT2.

Article Title: Acetylation of ELMO1 correlates with Rac1 activity and colorectal cancer progress.
Article Snippet: Acetylation, a critical regulator of diverse cellular processes, holds significant implications in various cancer contexts.. Further understanding of the acetylation patterns of key cancer-driven proteins is crucial for advancing therapeutic strategies in cancer treatment.. This study aimed to unravel the acetylation patterns of Engulfment and Cell Motility Protein 1 (ELMO1) and its relevance to the pathogenesis of colorectal cancer (CRC).

Immunoprecipitation:

Chromatin Immunoprecipitation:

Article Title: Molecular regulatory mechanism of the SIRT2/NF-κB p65 signaling pathway in ferroptosis-promoted spinal cord injury repair
Article Snippet: .. Each experiment is repeated three times.Use the following primary antibodies at specified dilutions: SIRT2 Polyclonal antibody (19655-1-AP, Proteintech, USA, dilution 1:5000)Anti-NF-kB p65 (acetyl K310) antibody [ EPR21781 ] - ChIP Grade (ab218533, Abcam, UK, dilution 1:1000)Anti-NF-kB p65 antibody [E379] (ab32536, Abcam, UK, dilution 1:1000)GPX4 Monoclonal antibody (67763-1-Ig, Proteintech, USA, dilution 1:5000)Anti-4 Hydroxynonenal antibody [HNEJ-2] (ab48506, Abcam, UK, dilution 1:1000)GAPDH (ab8245, Abcam, UK, dilution 1:2000). ..

Article Title: Molecular regulatory mechanism of the SIRT2/NF-κB p65 signaling pathway in ferroptosis-promoted spinal cord injury repair.
Article Snippet: .. Each experiment is repeated three times.Use the following primary antibodies at specified dilutions: SIRT2 Polyclonal antibody (19655-1-AP, Proteintech, USA, dilution 1:5000)Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade (ab218533, Abcam, UK, dilution 1:1000)Anti-NF-kB p65 antibody [E379] (ab32536, Abcam, UK, dilution 1:1000)GPX4 Monoclonal antibody (67763-1-Ig, Proteintech, USA, dilution 1:5000)Anti-4 Hydroxynonenal antibody [HNEJ-2] (ab48506, Abcam, UK, dilution 1:1000)GAPDH (ab8245, Abcam, UK, dilution 1:2000). ..

Cell-Signaling:

Article Title: S‐ketamine Alleviates Neuroinflammation and Attenuates Lipopolysaccharide‐Induced Depression Via Targeting SIRT2
Article Snippet: S‐KET (86901445003581) was purchased from Jiangsu Hengrui Pharmaceuticals Co., Ltd. Racemic ketamine was provided by the Department of Public Security Jilin Province. .. R‐KET was resolved from racemic ketamine (Chiral resolution of racemic ketamine in Scheme , Supporting Information).The antibodies with the indicated dilutions were as follows: SIRT2 polyclonal antibody (Proteintech, 19655‐1‐AP, 1:200); SIRT2 monoclonal antibody (Proteintech, 66410‐1‐Ig, 1:3000); β‐actin (Proteintech, 66009‐1‐Ig, 1:10000); Rabbit IgG (Bioworld, BD0051, 1:1000); HRP‐conjugated Affinipure Goat Anti‐Mouse IgG (H+L) (Proteintech, SA00001‐1, 1:2000), HRP‐conjugated Affinipure Goat Anti‐Rabbit IgG (H+L) (Proteintech, SA00001‐2, 1:3000); Alexa Fluor 488 Goat Anti‐Mouse IgG H&L (Abcam, ab150113, 1:200); NF‐κB p65 (Cell Signaling Technology, 8242, 1:1000); Acetyl‐NF‐κB p65 (Lys310) (Cell Signaling Technology, 12629, 1:1000); Iba‐1 (Abcam, ab283319, 1:200); VeriBlot for IP Detection (Abcam, ab131366, 1:1000). .. C57BL/6J male mice (7‐8 weeks old, weighing 22–24 g) were sourced from Beijing Vital River Laboratory Animal Technology Co., Ltd. Mice were maintained in a standard environment with a temperature range of 22–24 °C, humidity levels of 40–60%, and a light cycle 12 h (8:00 AM to 8:00 PM).

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